人源卵巢微血管内皮细胞

Angiogenesis is critical for many physiological processes including organ development and tissue repair. In human ovaries, angiogenesis is known to be associated with the development of follicles and the formation of the corpus luteum. A complex vascular network is formed within the thecal cell layer during follicular growth, and rapid neovascularization occurs toward the granulosa cell layer after ovulation. Endothelial cells from different organs, as well as from large and small vessels of the same organ, have specialized properties. The endothelial cells prepared from the specific microvasculature of the ovarian follicle are an ideal in vitro model for the study of the angiogenesis and physiology of the female reproductive system.

HOMEC from ScienCell Research Laboratories are isolated from human ovary. HOMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HOMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HOMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HOMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HOMEC in vitro.

血管生成对于许多生理过程至关重要，包括器官发育和组织修复。在人类卵巢中，血管生成与卵泡发育以及黄体形成密切相关。在卵泡生长过程中，卵泡膜细胞层内会形成复杂的血管网络；排卵后，向颗粒细胞层方向会发生快速的新生血管形成。不同器官来源的内皮细胞，以及同一器官中来自不同大小血管的内皮细胞，均具有特异性的生物学特性。从卵巢卵泡特定微血管中制备的内皮细胞，是研究女性生殖系统血管生成机制和生理功能的理想体外研究模型。

ScienCell Research Laboratories 提供的人源卵巢微血管内皮细胞（HOMEC）分离自人源卵巢组织。HOMEC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HOMEC 经采用针对 vWF/凝血因子 VIII（vWF/Factor VIII）和/或 CD31（PECAM1）的特异性抗体进行免疫荧光鉴定。HOMEC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HOMEC 可保证进一步扩增 10 个群体倍增周期。

推荐培养基

建议使用内皮细胞培养基（ECM，产品编号 #1001）对 HOMEC 进行体外培养。

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