人卵巢成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They synthesize components of the extracellular matrix, maintain the structural integrity of connective tissues, and actively participate in wound healing. Accumulating evidence indicates that ovarian fibroblasts can be induced and activated by ovarian cancer cells to secrete a variety of growth factors, chemokines, collagens, and matrix-modifying enzymes to establish a pro-tumorigenic, anti-immunogenic niche for cancer progression and metastasis. These results suggest that fibroblasts play a stimulatory functional role in cancer development and invasive behavior.

HOF from ScienCell Research Laboratories are isolated from human ovary. HOF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HOF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HOF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HOF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HOF in vitro.

成纤维细胞是一类来源于胚胎中胚层的间充质细胞。它们可合成细胞外基质成分，维持结缔组织的结构完整性，并在伤口愈合过程中发挥重要作用。越来越多的研究表明，卵巢成纤维细胞可受到卵巢癌细胞的诱导和激活，分泌多种生长因子、趋化因子、胶原蛋白以及基质调控酶，从而建立有利于肿瘤发生、免疫抑制的微环境，促进癌症进展和转移。这些研究结果表明，成纤维细胞在癌症发生发展及侵袭过程中具有重要的促进作用。

ScienCell Research Laboratories 提供的人卵巢成纤维细胞（HOF）分离自人卵巢组织。HOF 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HOF 通过其典型的梭形细胞形态以及使用纤维连接蛋白（fibronectin）特异性抗体进行免疫荧光染色鉴定。HOF 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HOF 可保证进一步扩增至少 15 次群体倍增。

推荐培养基

建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HOF 进行体外培养。

Search for a Certificate of Analysis by Lot Number below:

No Publication available at this time.