人源子宫肌层微血管内皮细胞
分离自人源子宫组织。人源子宫肌层微血管内皮细胞（HMMEC） 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 105 个细胞，体积为 1 ml。
Myometrial microvascular endothelial cells (MMEC) are located in the myometrium between the endometrium and the serosa of the uterus and exhibit typical cobblestone morphology. During pregnancy, angiogenesis occurs through MMEC proliferation in order to support the hypertrophy of the myometrial smooth muscle cell layer. Significant evidence also indicates that endothelial cell dysfunction in the myometrium may contribute to the development of preeclampsia. In addition, MMEC proliferation and angiogenesis may play a role in the formation of leiomyomata. Studies have shown that MMEC express the oxytocin receptor, which may promote vasodilation in the microvasculature of the myometrium. Human MMEC are a useful in vitro model to elucidate the mechanisms of angiogenesis and to develop treatments for female reproductive tract disorders.
HMMEC from ScienCell Research Laboratories are isolated from human uterus. HMMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HMMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HMMEC in vitro.
子宫肌层微血管内皮细胞（MMEC）位于子宫内膜与浆膜之间的子宫肌层内，具有典型的鹅卵石样形态。在妊娠期间，为支持子宫肌层平滑肌细胞层的肥大，MMEC 通过增殖促进血管生成。大量研究证据表明，子宫肌层内皮细胞功能障碍可能参与子痫前期的发生。此外，MMEC 的增殖及血管生成也可能在子宫平滑肌瘤的形成过程中发挥作用。研究显示，MMEC 表达催产素受体（oxytocin receptor），该受体可能促进子宫肌层微血管的血管舒张。人源 MMEC 是阐明血管生成机制及开发女性生殖道疾病治疗方法的理想体外研究模型。
ScienCell Research Laboratories 提供的人源子宫肌层微血管内皮细胞（HMMEC）分离自人源子宫组织。HMMEC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 105 个细胞，体积为 1 ml。HMMEC 经采用针对 vWF/凝血因子 VIII（vWF/Factor VIII）和/或 CD31（PECAM1）的特异性抗体进行免疫荧光鉴定。HMMEC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HMMEC 可保证进一步扩增 10 个群体倍增周期。
推荐培养基
建议使用内皮细胞培养基（ECM，产品编号 #1001）对 HMMEC 进行体外培养。
| 目录编号 | 7000 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HMMEC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，立即将细胞从干冰转移到液氮中，并在液氮中保存，直到需要进行实验。 |
| 运输 | 干冰。 |
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