人食管微血管内皮细胞

Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that human esophageal microvascular endothelial cells (HEsMEC) exhibit a strong inflammatory immune response to LPS, cytokines, and pH exposure, thus playing a critical regulatory role in esophageal inflammation. When exposed to acidic pH, HEsMEC express certain proteins, such as VCAM-1, but not others, such as ICAM-1. This expression pattern could play a role in the cellular response triggered by gastroesophageal reflux disease. HEsMEC cultures have enabled scientists to observe leukocyte binding, upregulation of cell adhesion molecules, and other cellular responses to LPS, cytokines and pH exposure and has shown that HEsMEC are intrinsically different from lower gastrointestinal endothelial cells.

HEsMEC from ScienCell Research Laboratories are isolated from human esophageal tissue. HEsMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HEsMEC are characterized by immunofluorescence with antibody specific to vWF/Factor VIII and/or CD31 (PECAM1). HEsMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEsMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HEsMEC in vitro.

排列于微血管内壁的内皮细胞在炎症过程中发挥关键的“守门员”作用，其能够募集循环中的免疫细胞进入组织及炎症病灶。研究表明，人食管微血管内皮细胞（HEsMEC）对脂多糖（LPS）、细胞因子以及 pH 变化表现出强烈的炎症免疫反应，因此在食管炎症调控过程中发挥重要作用。当暴露于酸性 pH 环境时，HEsMEC 可表达某些特定蛋白（如 VCAM-1），但不表达其他蛋白（如 ICAM-1）。这种差异性表达模式可能参与胃食管反流病（GERD）诱导的细胞反应过程。HEsMEC 细胞培养体系使科研人员能够观察白细胞黏附、细胞黏附分子上调，以及细胞对 LPS、细胞因子和 pH 刺激产生的其他反应，并发现 HEsMEC 与下消化道内皮细胞在生物学特性上存在本质差异。

ScienCell Research Laboratories 提供的人食管微血管内皮细胞（HEsMEC）分离自人食管组织。HEsMEC 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HEsMEC 通过使用针对 vWF/Ⅷ因子（Factor VIII）和/或 CD31（PECAM1）的特异性抗体进行免疫荧光染色鉴定。HEsMEC 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HEsMEC 可保证进一步扩增至少 10 次群体倍增。

推荐培养基

建议使用内皮细胞培养基（Endothelial Cell Medium，ECM，产品编号 #1001）对 HEsMEC 进行体外培养。