人源子宫内膜微血管内皮细胞

Angiogenesis is critical for many physiological processes including organ development, tissue repair, and the female reproductive tract. Human endometrial microvascular endothelial cells (HEMEC) are located in the inner mucous membrane of the uterus known as the endometrium. HEMEC are involved in endometrial angiogenesis during the menstrual cycle with the rapid growth and shedding of the endometrium. Angiogenesis in the endometrium occurs through the non-sprouting mechanisms intussusception and vessel elongation. Studies have indicated that elevated HEMEC proliferation in the endometrium can lead to disorders such as endometriosis and menorrhagia. Cultured HEMEC are a useful model to elucidate the mechanisms of normal and pathological angiogenesis and develop treatments for female reproductive tract disorders.

HEMEC from ScienCell Research Laboratories are isolated from human uterus. HEMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HEMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HEMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HEMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HEMEC in vitro.

血管生成对于器官发育、组织修复以及女性生殖系统等多种生理过程至关重要。人源子宫内膜微血管内皮细胞（HEMEC）位于子宫内层黏膜——子宫内膜中。在月经周期中，随着子宫内膜的快速增殖和周期性脱落，HEMEC 参与子宫内膜血管生成。子宫内膜血管生成主要通过非出芽性机制进行，包括血管内分隔形成（intussusception）和血管延长（vessel elongation）。研究表明，子宫内膜中 HEMEC 的异常增殖可导致子宫内膜异位症和月经过多等疾病。培养的人源 HEMEC 是阐明正常及病理性血管生成机制，并开发女性生殖道疾病治疗策略的理想体外研究模型。

ScienCell Research Laboratories 提供的人源子宫内膜微血管内皮细胞（HEMEC）分离自人源子宫组织。HEMEC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HEMEC 经采用针对 vWF/凝血因子 VIII（vWF/Factor VIII）和/或 CD31（PECAM1）的特异性抗体进行免疫荧光鉴定。HEMEC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HEMEC 可保证进一步扩增 10 个群体倍增周期。

推荐培养基

建议使用内皮细胞培养基（ECM，产品编号 #1001）对 HEMEC 进行体外培养。

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