人源胎儿真皮成纤维细胞（丝裂霉素 C 处理）

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrixremodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.

HDF-f-mt from ScienCell Research Laboratories are isolated from fetal human skin. HDF-f-mt are cryopreserved at passage one and delivered frozen. Each vial contains > 5 x 10⁵ cells in 1 ml volume. HDF-f-mt are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-f-mt are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-f-mt are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HDF-f-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-f-mt in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞是最容易进行体外培养的细胞类型之一，因此被广泛应用于各种细胞和分子生物学研究。其较强的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。通常情况下，成纤维细胞可分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的非刚性细胞外基质。有研究表明，不同器官来源的成纤维细胞在生物学特性上存在内在差异。在创伤愈合过程中，真皮成纤维细胞可由增殖和迁移状态转变为收缩及基质重塑状态。此外，在炎症刺激响应下，真皮成纤维细胞还可分泌大量透明质酸。

ScienCell Research Laboratories 提供的人源胎儿真皮成纤维细胞（丝裂霉素 C 处理型，HDF-f-mt）分离自人源胎儿皮肤组织。HDF-f-mt 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HDF-f-mt 根据其梭形细胞形态以及采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HDF-f-mt 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HDF-f-mt 可继续进行培养；然而，由于丝裂霉素 C 处理会抑制细胞进一步增殖，因此不建议用于细胞扩增。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HDF-f-mt 进行体外培养。