人源宫颈上皮细胞

The cervix is the lower portion of the uterus and functions to promote fertility and protect the female reproductive tract and fetus from potential pathogens. In addition, the cervix plays a critical role in childbirth by effacing and dilating to allow the baby to pass out of the uterus. Cervical epithelial cells (CerEpiC), which line the cervix, maintain structural integrity and limit the passage of molecular and cellular substances into the cervix using intercellular junctions. Studies have shown that patients infected with human papillomavirus (HPV) causes inflammation of the epithelial cell layer which can lead to the development of cervical carcinoma. Primary human CerEpiC (HCerEpiC) are a useful in vitro model for studying the pathophysiology of cervical polyps, HPV, and cervical cancer.

HCerEpiC from ScienCell Research Laboratories are isolated from human uterus. HCerEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCerEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HCerEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCerEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Cervical Epithelial Cell Medium (CerEpiCM, Cat. #7061) for culturing HCerEpiC in vitro.

宫颈是子宫的下部结构，在促进生育以及保护女性生殖系统和胎儿免受潜在病原体侵害方面发挥重要作用。此外，宫颈在分娩过程中通过消退和扩张，使胎儿能够顺利通过子宫，是分娩过程中的关键结构。覆盖于宫颈表面的宫颈上皮细胞（CerEpiC）通过细胞间连接维持组织结构完整性，并限制分子及细胞物质进入宫颈。研究表明，感染人乳头瘤病毒（HPV）的患者可引起上皮细胞层炎症，并可能进一步导致宫颈癌的发生。原代人源宫颈上皮细胞（HCerEpiC）是研究宫颈息肉、HPV 感染以及宫颈癌病理机制的有价值的体外研究模型。

ScienCell Research Laboratories 提供的人源宫颈上皮细胞（HCerEpiC）分离自人源宫颈组织。HCerEpiC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HCerEpiC 经采用针对细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）的特异性抗体进行免疫荧光鉴定。HCerEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HCerEpiC 可保证进一步扩增 10 个群体倍增周期。

推荐培养基

建议使用宫颈上皮细胞培养基（CerEpiCM，产品编号 #7061）对 HCerEpiC 进行体外培养。

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