人膀胱微血管内皮细胞

The bladder is a hollow ball, with the wall of the bladder composed of the serosa, muscularis, submucosa, musculris mucosa, and lamina propria. The endothelial cells lining the microvasculature of the circulatory system in the bladder are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues. Endothelial cells in the bladder wall stimulated with thrombin or tryptase causes phospholipid metabolite production and may contribute to the inflammatory process in interstitial cystitis. HBdMEC provide a useful in vitro model to study the role of endothelial cells in physiological and inflammatory conditions.

HBdMEC from ScienCell Research Laboratories are isolated from human bladder tissue. HBdMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBdMEC are characterized by immunofluorescence with antibodies specific to VWF/Factor VIII and/or CD31 (PECAM1). HBdMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdMEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HBdMEC.

膀胱是一个中空的球状器官，其壁由浆膜层、肌层、黏膜下层、黏膜肌层和固有层构成。膀胱循环系统微血管内皮细胞可通过募集循环血液中的免疫细胞进入组织，在炎症过程中发挥重要的“守门人”作用。膀胱壁内皮细胞受到凝血酶或类胰蛋白酶刺激后，会促进磷脂代谢物的产生，并可能参与间质性膀胱炎的炎症过程。人膀胱微血管内皮细胞（HBdMEC）可作为有价值的体外模型，用于研究内皮细胞在生理和炎症状态下的作用。

ScienCell Research Laboratories 的人膀胱微血管内皮细胞（HBdMEC）分离自人膀胱组织。HBdMEC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。HBdMEC 采用针对血管性血友病因子（VWF）/凝血因子 VIII 和/或 CD31（PECAM1）的特异性抗体进行免疫荧光鉴定。HBdMEC 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HBdMEC 可进一步扩增 15 个群体倍增（population doublings）。

推荐培养基

推荐使用内皮细胞培养基（Endothelial Cell Medium，ECM，产品编号：1001）培养 HBdMEC。

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