人脂肪微血管内皮细胞

Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are located. Adipose tissue is unique because it has the capacity to continually grow throughout adult life. Thus, it has a high level of angiogenesis to provide the extensive vascularization required for adipose tissue. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. At the same time, microvascular endothelial cell growth is stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationship between microvascular endothelial cells and preadipocytes during tissue development.

HAMEC from ScienCell Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HAMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HAMEC.

微血管内皮细胞衬覆于微血管内壁，参与血管生成、凝血、淋巴细胞运输以及炎症反应等多种重要生物学过程。不同组织和器官来源的微血管内皮细胞具有各自特异的细胞学特征和生物学功能。脂肪组织具有在成年期持续生长和扩增的能力，因此需要高度活跃的血管生成过程，以满足其丰富血管网络形成的需求。研究表明，血管生成先于脂肪生成发生，提示微血管内皮细胞可调控前脂肪细胞的增殖。同时，脂肪细胞分泌的血管内皮生长因子（VEGF）能够促进微血管内皮细胞生长，说明在组织发育过程中，微血管内皮细胞与前脂肪细胞之间存在复杂的旁分泌调控关系。

ScienCell Research Laboratories 提供的人脂肪微血管内皮细胞（HAMEC）分离自人脂肪组织。HAMEC 以第 1 代（P1）状态进行冻存，并以冷冻形式运输。每支冻存管含有 >5 × 10⁵ 个细胞，体积为 1 ml。HAMEC 经免疫荧光检测，表达 vWF/Factor VIII 和/或 CD31（PECAM1）等内皮细胞特异性标志物。经检测，HAMEC 不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌。在 ScienCell Research Laboratories 推荐的培养条件下，保证 HAMEC 可继续扩增至少 10 个群体倍增。

推荐培养基

建议使用内皮细胞培养基（Endothelial Cell Medium，ECM，产品编号：1001）培养 HAMEC。