GeneQuery™ 人Wnt信号通路qPCR阵列试剂盒

ScienCell's GeneQuery™ Human Wnt Signaling Pathway qPCR Array Kit (GQH-WNT) is designed to facilitate gene expression profiling of 88 key genes involved in Wnt signaling transduction. The Wnt signaling pathway is well known for its role in carcinogenesis and embryonic development. This kit focuses on canonical Wnt signaling. Brief examples of how included genes may be grouped according to purpose are shown below:

• Ligands: all known WNTs
• Receptors: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6
• Receptor transducers:  DVL1, DVL2, DVL3, CSNK1E, MARK1
• Transcription factors: CTNNB1, TCF7L1, TCF7L2, LEF1, PYGO1
• Regulatory elements: NLK, NKD1, AES, SFRP1, LRP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

ScienCell的GeneQuery™ 人Wnt信号通路qPCR阵列试剂盒（GQH-WNT）可用于对参与Wnt信号转导的88个关键基因进行表达谱分析。Wnt信号通路在肿瘤发生及胚胎发育中具有重要作用。本试剂盒重点关注经典Wnt信号通路。以下为部分基因按功能分类的示例：

• 配体：所有已知的WNT家族成员
• 受体：FZD1-10、SMO、LRP5/6、RSPO1-4、LGR4-6
• 受体信号转导蛋白：DVL1、DVL2、DVL3、CSNK1E、MARK1
• 转录因子：CTNNB1、TCF7L1、TCF7L2、LEF1、PYGO1
• 调控因子：NLK、NKD1、AES、SFRP1、LRP1

GeneQuery™ qPCR阵列试剂盒采用96孔板预装格式，可直接用于qPCR实验。每个孔中均含有一组特异性引物，用于识别并高效扩增对应目标基因的cDNA。精心设计的引物确保：(i) 在qPCR分析中具有最佳退火温度为65°C（在2 mM Mg²⁺且不添加DMSO条件下）；(ii) 除非另有说明，可识别目标基因的所有已知转录变体；(iii) 仅扩增单一目标基因。每组引物均通过qPCR熔解曲线分析及凝胶电泳验证。