GeneQuery™ Human Skeletal Muscle Contraction and Muscular Dystrophies qPCR Array Kit

ScienCell's GeneQuery™ Human Skeletal Muscle Contraction and Muscular Dystrophies qPCR Array (GQH-SKC) profiles 88 key genes involved in the contraction of skeletal muscle. There are three major muscle types in the human body: skeletal, cardiac, and smooth. Skeletal muscle is a striated muscle that relaxes and contracts to generate movement and force in the body. Of the three main muscle types, it is the only one under voluntary control. Neuromuscular diseases involving skeletal muscle dysfunction are very diverse and range from myopathies to muscular dystrophies. Below are brief examples of how included genes may be grouped according to their function in skeletal muscle biology:

• Myogenesis: MYOD1, MYOG, PAX3, UTRN, MYF6, MDTN
• Contractility: MYH2, SLC2A4, LMNA, DYSF, APT2A1
• Sarcomere Assembly : NEB, TRIM63, TTN, CAPN3, ACTA1
• Muscle Atrophy: NOS2, TRIM63, FOXO1, MAPK8, AKT1
• Muscular Dystrophy Development: POMT2, FKRP, ANO5, FRG1, SGCB

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.