GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit

ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

• Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
• Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
• Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
• Metabolism: PKM, STC2, DHX35, DDX11, ERO1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.