GeneQuery™ Human Cell Junctions qPCR Array Kit

ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance. This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring. Brief examples of how included genes may be categorized are shown below:

• Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
• Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
• Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
• Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.