GeneQuery™ HLA-B PCR+Sanger SBT 分型试剂盒

The human leukocyte antigen (HLA) complex is a gene complex located on the short arm of human chromosome 6. The HLA complex is the human version of the major histocompatibility complex (MHC) which helps the immune system distinguish host cells from invaders. It is the most polymorphic locus in the human genome, and has been implicated in many pathologies including autoimmunity disorders and cancer.

GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit (SBT-B) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

SBT-B kit contains a ScienCell proprietary HLA-B amplification primer set (B_ampF primer mix and B_ampR primer mix; Cat #GK892a) that can amplify a region in the human genome that covers exons 2-4 of HLA-B gene, and 6 sequencing primer mixes (B_2F, B_2R, B_3F, B_3R, B_4F, and B_4R; Cat #s GK892b-g) that can be used in the following Sanger sequencing of the PCR product. Please refer to Figure 2 for the binding location of the primers on the human genome. Each GeneQuery™ HLA typing kit contains a human Genomic DNA Reference (GDR) sample with known HLA alleles (Cat #GK892r). The GDR can be used to monitor the quality of the reagents and the instruments. The HLA-B alleles in GK892r are HLA-B*08:01 and HLA-B*18:01.

Primer binding locations on the human genome.

Additional Materials Required (Materials Not Included in Kit)

人类白细胞抗原（Human Leukocyte Antigen，HLA）复合体位于人类 6 号染色体短臂，是人类主要组织相容性复合体（Major Histocompatibility Complex，MHC）的组成部分，可帮助免疫系统识别自身细胞与外来入侵物。HLA 是人类基因组中多态性最高的基因区域之一，与多种疾病密切相关，包括自身免疫性疾病和肿瘤等。

GeneQuery™ HLA-B PCR+Sanger SBT 基因分型试剂盒（SBT-B）采用基于序列的分型（Sequence-Based Typing，SBT）技术，这是目前 HLA 分型的金标准。该方法首先对 HLA 基因进行 PCR 扩增，然后对扩增产物进行 Sanger 测序。获得的测序结果可通过 SOAPTyping 软件（BGI Genomics）与 HLA 等位基因数据库进行比对分析，从而获得样本的四位（4-digit）HLA-B 基因型。本试剂盒结合 SOAPTyping 软件，可同时解析来自父母双方的两条单倍型（haplotypes）。

SBT-B 试剂盒包含 ScienCell 专有的 HLA-B 扩增引物组（B_ampF 和 B_ampR，引物混合液；产品编号：GK892a），可扩增覆盖 HLA-B 基因第 2–4 外显子（Exons 2–4）的目标区域；同时配备 6 种测序引物混合液（B_2F、B_2R、B_3F、B_3R、B_4F 和 B_4R；产品编号：GK892b–GK892g），用于后续 PCR 扩增产物的 Sanger 测序。各引物在人类基因组上的结合位置请参见图 2。

每套 GeneQuery™ HLA 分型试剂盒均配有人基因组 DNA 参考品（Genomic DNA Reference，GDR；产品编号：GK892r），其 HLA 等位基因型已知，可用于监测试剂性能和仪器运行状态。GK892r 所含 HLA-B 等位基因型为 HLA-B*08:01 和 HLA-B*18:01。

引物在人类基因组上的结合位置

所需其他材料（试剂盒未提供）