GeneQuery™ Human Hippo Signaling Pathway qPCR Array Kit

ScienCell's GeneQuery™ Human Hippo Signaling Pathway qPCR Array Kit (GQH-HIP) is designed to profile 88 key genes involved in the Hippo Signaling Pathway. The Hippo signaling pathway is crucial for regulating cell proliferation, apoptosis, and stem cell self-renewal. It plays a pivotal role in controlling cellular processes through a kinase cascade that ultimately modulates the activity of transcription factors. This pathway influences gene expression patterns and cellular outcomes, including growth suppression and tissue homeostasis. ScienCell's GeneQuery™ Human Hippo Signaling Pathway qPCR Array Kit provides a comprehensive platform for investigating the expression profiles of key genes involved in Hippo signaling. Brief examples of how included genes may be grouped according to function are shown below:

• Receptor: FZD1, FZD2, FZD3, FZD4, FZD5, FZD6
• Regulatory Element: YAP1, WWTR1, CCND1, ID2
• Transcription factor: MYC, SMAD2, TP73

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC)

GeneQuery™ qPCR array kits are qPCR-ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.