Absolute Human Kringle Repeats Quantification qPCR Assay Kit

Lipoprotein(a), or Lp(a), is one subclass of lipoproteins. It is the defining protein component of Lp(a) particles. Plasma Lp(a) concentrations have been associated with atherosclerotic and cardiovascular diseases. It has been reported that plasma Lp(a) concentrations are hereditary and have been linked to the highly polymorphic LPA gene located on human chromosome 6. LPA gene contains 10 types of kringles, namely kringle-IV types 1-10 (KIV1-10), that are highly homologous in sequence. Moreover, one type of kringle, KIV2, exhibits a high level of copy number variation. The number of KIV2 repeats can vary from 5 to over 50 among human populations. ScienCell's Absolute Human Kringle Repeats Quantification qPCR Assay Kit (AHKRQ) is designed to directly measure the number of kringle repeats of a human cell sample. The kringle repeats (KRG) primer set recognizes and amplifies all kringle IV repeats, including KIV1-10. The single copy reference (SCR) primer set recognizes and amplifies a region on human chromosome 17, and serves as reference for data normalization. The reference DNA sample with a 1:1 ratio of kringle repeats copies to SCR copies serves as a reference for calculating the number of kringle repeats of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.