ScienCell's Leading DNA and RNA Purification Kits - ScienCell Research Laboratories

What is DNA/RNA Isolation?

DNA/RNA isolation, also referred as nucleic acid purification, is the process of extracting and purifying DNA or RNA from biological samples like cells, tissues, and body fluids.

The purpose of DNA/RNA isolation is to obtain pure nucleic acid samples free from contaminants and inhibitors. These contaminants can include proteins, lipids, carbohydrates, and other cellular debris that can interfere with downstream applications.

Isolation kits, such as those offered by ScienCell, provide standardized and efficient methods for DNA/RNA isolation. These kits typically use spin column-based technologies that utilize specific buffers and reagents to lyse cells, bind nucleic acids to a membrane, wash away contaminants, and finally elute purified DNA or RNA.

Here are just a few examples of how purified DNA/RNA can be used in research and clinical settings:

Polymerase Chain Reaction (PCR): Amplifying specific DNA sequences for various applications, including gene cloning, disease diagnosis, and forensic analysis.
Sequencing: Determining the order of nucleotides in DNA or RNA, which is fundamental for understanding gene function, identifying mutations, and studying evolution.
Gene expression analysis: Measuring the levels of RNA transcripts to study gene regulation, cellular responses, and disease mechanisms.
Genotyping: Identifying genetic variations that can be associated with disease susceptibility, drug response, or ancestry.
Cloning: Inserting DNA fragments into vectors for gene expression, protein production, or gene therapy.

The availability of high-quality DNA/RNA isolation kits has revolutionized molecular biology research and diagnostics by providing reliable, efficient, and user-friendly methods for obtaining pure nucleic acid samples. This has paved the way for countless scientific advancements and continues to drive innovation in the field.

ScienCell offers a variety of kits for storage and purification of DNA and RNA from a variety of sources. Here’s a closer look at ScienCell’s DNA and RNA isolation and storage solutions.

StableRNA Storage Solution

ScienCell’s StableRNA Storage Solution is a reagent that stabilizes and protects nucleic acids in various specimen types, including animal tissues, body fluids, plant tissues, mammalian cells, bacteria, and yeast.
This solution allows researchers to avoid immediate processing or freezing of fresh specimens.
Samples can be stored at room temperature for up to 7 days or indefinitely at -20°C or below.
StableRNA Storage Solution is compatible with all of ScienCell’s purification kits, such as the Viral RNA Isolation Kit and the DualPrep DNA/RNA Isolation Kit.

DualPrep DNA/RNA Isolation Kit

The DualPrep DNA/RNA Isolation Kit allows for the simultaneous purification of genomic DNA and total RNA from a single sample.
This kit uses a series of spin columns to selectively isolate both DNA and RNA.
The purified DNA and RNA can then be used for a variety of downstream applications, such as PCR, Southern blotting, genotyping, cDNA synthesis, microarray analysis, RT-qPCR, RNA-Seq, and Northern blotting.
The kit includes various buffers, spin columns, and nuclease-free water.
StableRNA Storage Solution is listed as a required material for use with the DualPrep DNA/RNA Isolation Kit, suggesting that samples may be stored in StableRNA prior to DNA/RNA extraction.

SpeeDNA Isolation Kit

The SpeeDNA Isolation Kit is designed for the rapid purification of genomic DNA from samples such as whole blood, plasma, serum, tissues, and cultured cells.
A key advantage of this kit is its speed, isolating DNA in less than 15 minutes.
The purified DNA is suitable for applications such as PCR, Southern blotting, and genotyping.

SpeeDNA Plasmid Miniprep Kit

The SpeeDNA Plasmid Miniprep Kit enables quick and easy purification of plasmid DNA from bacterial cells.
This kit uses a spin-column based method and can recover up to 25 µg of high-quality plasmid DNA.
The purified plasmid DNA can be used for various downstream applications, including restriction enzyme digestion, ligation, transformation, transfection, PCR, and DNA sequencing.

SpeeDNA Gel Extraction Kit

The SpeeDNA Gel Extraction Kit is used to isolate DNA fragments from agarose gels.
This kit uses a spin-column based method that allows for recovery rates of up to 95%.
The purified DNA can be utilized for various downstream applications, including PCR, restriction digestion, in situ hybridization, and cloning.

SpeeDNA PCR Purification Kit

The SpeeDNA PCR Purification Kit is designed to purify DNA fragments from PCR reactions.
This kit uses a spin-column based method and can recover up to 95% of the DNA.
The purified DNA is suitable for applications such as PCR, restriction digestion, in situ hybridization, and cloning.

Viral RNA Isolation Kit

The Viral RNA Isolation Kit is designed for the purification of viral RNA from various samples, including cell culture media, plasma, serum, saliva, and swab specimens.
The kit employs a spin column-based purification method and can recover over 80% of RNA longer than 200 nucleotides.
The purified RNA can be used for downstream applications such as RT-PCR and next-generation sequencing15.

RNase Inhibitor, Murine

RNase Inhibitor, Murine (rmRnh1) is a recombinant protein that inactivates a broad range of RNases.
This inhibitor is particularly useful in applications where RNase contamination might pose a problem, such as RT-PCR, cDNA synthesis, in vitro transcription, and RNA labeling reactions.
It is important to note that rmRnh1 does not affect the activity of enzymes commonly used in RNA preparation and analysis, including Taq DNA polymerase, phage RNA polymerases, and M-MuLV reverse transcriptase.

ScienCell offers a range of products for the isolation, purification, and storage of DNA and RNA. These products are designed for various applications and sample types, making them valuable tools for molecular biology research.

ScienCell’s Leading DNA and RNA Purification Kits