How would you measure telomere length?

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.

ScienCell exclusively offers qPCR-based assay kits for the accurate, simple, and fast telomere length quantification of various species samples:

Absolute

Includes a reference gDNA sample with known telomere length. This kit can be used to calculate a sample’s actual telomere length in kb.

For more information, please visit

▪ Absolute Human Telomere Length Quantification qPCR Assay Kit (Cat.# 8918)
▪ Absolute Mouse Telomere Length Quantification qPCR Assay Kit (Cat.# M8918)
▪ Absolute Rat Telomere Length Quantification qPCR Assay Kit (Cat.#R8918)
▪ Absolute Bovine Telomere Length Quantification qPCR Assay Kit (Cat.#B8918)
▪ Absolute Pig Telomere Length Quantification qPCR Assay Kit (Cat.#P8918)

Relative

NO reference gDNA sample included. Can be used to compare the telomere length of samples in fold change.

For more information, please visit

▪ Relative Human Telomere Length Quantification qPCR Assay Kit (Cat.# 8908)
▪ Relative Mouse Telomere Length Quantification qPCR Assay Kit (Cat.# M8908)
▪ Relative Rat Telomere Length Quantification qPCR Assay Kit (Cat.# R8908)

PRODUCT CHARACTERISTICS:

Unique: specially modified primers only available at ScienCell
Accurate: highly specific and qPCR efficiency close to 100%
Reliable: NO detectable primer dimer formation after 28-30 cycles
Informative: telomere length expressed in kilobases (Cat. #8918 only)
Sensitive: as low as 0.1 ng of gDNA required
Convenient: NO special treatment of gDNA necessary
Rapid: results can be acquired within 1 hour
Robust: suitable for a high-throughput setup

Figure 1. qPCR amplification curves using serially diluted telomere repeats as template.
Figure 2. Derivation of qPCR efficiency of Telomere primer set.

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