Author Archives: Yongjiang Li

About Yongjiang Li

Yongjiang Daniel Li, PhD Research Scientist, Research and Development ScienCell Research Laboratories 6076 Corte Del Cedro Carlsbad, CA 92011 Ph: (760) 602-8549 ext.257 yli@ScienCellonline.com https://www.ScienCellonline.com

Value of qPCR in the Next Generation Sequencing Era

qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.

pseudogene timeline

Hidden Treasure in “Junk DNA”

3 Reasons Why You Should Take “Pseudogenes” Seriously in Your Research Protein-coding DNA, or “functional” genes, only accounts for up to 2% in the human genome. Until recently, the remaining… Read more »

Comparison of Cell Viability, Proliferation and Apoptosis Assays

To obtain reliable data, assessing the health of cultured cells prior to any assays is essential. Furthermore, many cell-based assays require quantification of cell growth. Cell health and growth can be determined by quantifying cell viability, proliferation, or apoptosis

6 tips to improve qPCR

6 Tips to improve qPCR (Part 3 of 3)

In this final installment on how to improve your qPCR gene expression profiling (see 6 Tips to Improve qPCR: part 1 and part 2 for previous discussion), we will discuss… Read more »

Figure 2. Preliminary results acquired by comparison of human brain astrocytes and human retinal astrocytes using GeneQuery™ Astrocyte Cell Biology Kit (Cat# GK009).

Pilot Study: Results Acquired within 2 hours with GeneQuery™ Astrocyte Cell Biology Kit

Gene expression analysis is critical for understanding the transcriptome profiles of primary cells and how they directly influence the cells’ functionality. In addition, the transcriptome profile can affect cell proliferation,… Read more »