As of April 10, 2020, the number of U.S. SARS-CoV-2 coronavirus cases surpassed 500,000 with a death toll near 19,000. For over a century, coronaviruses were thought to only cause… Read more »
qPCR is a powerful tool for quantification of gene expression levels and copy number variation. Despite the advances in next-generation sequencing (NGS), qPCR still serves as the “gold standard” for… Read more »
Cancer immunotherapy is one promising cancer treatment option whereby the host’s own immune system is used to treat cancer. The therapy works by either stimulating certain immune activities, or counteracting… Read more »
Aging and Telomere Length Quantification by qPCR Aging is a time-dependent decline of the body’s functional capabilities and is an inevitable course of life (as shown in the image below,… Read more »
qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.
3 Reasons Why You Should Take “Pseudogenes” Seriously in Your Research Protein-coding DNA, or “functional” genes, only accounts for up to 2% in the human genome. Until recently, the remaining… Read more »
To obtain reliable data, assessing the health of cultured cells prior to any assays is essential. Furthermore, many cell-based assays require quantification of cell growth. Cell health and growth can be determined by quantifying cell viability, proliferation, or apoptosis
In this final installment on how to improve your qPCR gene expression profiling (see 6 Tips to Improve qPCR: part 1 and part 2 for previous discussion), we will discuss… Read more »
In this next installment on how to improve your qPCR gene profiling (see 6 Tips to Improve qPCR for previous discussion), we would like to discuss the limitations of melting… Read more »
Gene expression analysis is critical for understanding the transcriptome profiles of primary cells and how they directly influence the cells’ functionality. In addition, the transcriptome profile can affect cell proliferation,… Read more »