Recombinant Human Granulocyte Macrophage Colony Stimulating Factor
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.
| Catalog No. | 102-03 |
|---|---|
| Product Code | rhGM-CSF |
| Country of Manufacture | United States or China |
| Size/Quantity | 5ug, 20ug, or 1 mg |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Dry ice. |
| Synonyms | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897 |
| AA Sequence | MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
| Source | Escherichia coli |
| Molecular Weight | Approximately 14.6 kDa, a single non-glycosylated polypeptide chain containing 128 amino acids. |
| Purity | >96% by SDS-PAGE and HPLC analyses. |
| Biological Activity | The ED50 was determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of >5Ă—10^5 IU/mg. |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2ÎĽm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/ÎĽg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
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