Mouse Embryonic Fibroblast-Conditioned Medium
MEF-cm is a liquid medium prepared from primary CF1 mouse embryonic fibroblasts treated with mitomycin C. The medium is sterile filtered and consists of one 100-mL bottle.
This product requires a minimum purchase of 10 bottles and a 3 week estimated time.
Please contact sales@sciencellonline.com to place an order.
Mouse Embryonic Fibroblast-Conditioned Medium (MEF-cm) is a serum free medium for the feeder independent culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. MEF-cm is an efficient alternative to MEF feeder cells, supporting the self-renewal of hPSC and maintaining pluripotency in the presence of basic FGF and hPSC qualified matrix (e.g., BD MatrigelTM matrix). ScienCell's MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. #M7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 ÎĽm filter. Before using MEF-cm for hPSC culture, please add fresh recombinant human basic FGF (4 ng/mL) (rhbFGF, Cat #104-02) into the medium to make the complete medium. The complete MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.
| Catalog No. | 5881 |
|---|---|
| Country of Manufacture | United States |
| Product Code | MEF-cm |
| Size/Quantity | 100 ml |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store MEF-cm at ≤ -20°C in a manual defrost freezer. Thaw the medium overnight at 2-8°C in the dark. Aliquot and store the unused portions at ≤ -°C. Long-term storage (>2 weeks) at 2-8°C is not recommended. Avoid repeated freeze-thaw |
| Shipping | Dry ice. |
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