Mouse Dermal Fibroblasts from C57BL/6
MDF are isolated from postnatal day 2 C57BL/6 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume.
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in different organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
MDF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. MDF are negative for mycoplasma, bacteria, yeast, and fungi. MDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MDF in vitro.
| Catalog No. | M2300-57 |
|---|---|
| Country of Manufacture | United States |
| Product Code | MDF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping | Dry ice. |
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