Monkeypox Virus Multiplex qPCR Detection Kit

Monkeypox virus (MPXV) is a zoonotic virus that causes monkeypox in humans and animals. The enveloped double-stranded DNA virus belongs to the Orthopoxvirus genus in the Poxviridae family with a genome size of around 190 kb. The Poxviridae family also includes vaccinia virus, variola virus, cowpox virus, and several animal-related poxviruses. There are two distinct genetic clades of MPXV: the Central African clade and the West African clade. The Central African clade is thought to be more transmissible and causes more severe disease.

ScienCell's Monkeypox Virus Multiplex Detection Kit (MPXVD) is designed to detect the presence of monkeypox virus in dry swab specimen samples or extracted DNA samples from wet swab specimen, cell lysate, body fluid and other biological specimen samples. The cell lysis buffer and enhancer (Cat #GQ400a and GQ400b) are included in the kit to extract DNA from dry swab specimen samples for direct qPCR. The multiplex primer/probe set component (Cat #7178MPP) contains 3 primer/probe sets: MP1-FAM, MP2-FAM, and HR-HEX. Among them, MP1- FAM and MP2-FAM target highly conserved regions of monkeypox viruses. The carefully designed MP1-FAM and MP2-FAM primer/probe set can specifically recognize both the Central African clade and the West African clade of MPXV, but will not interact with other Poxviridae family viruses, including vaccinia virus, variola virus, and cowpox virus. HR-HEX primer/probe set targets human B2M gene and serves as a control to assess specimen quality. In addition, a non-infectious positive control (Cat #7178-Pos) and nuclease-free water (Cat #MB6048b-1) are included in the kit. The positive control (Cat #7178-Pos) consists of non-infectious viral DNA fragments spiked into human dermal fibroblast cells and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation.

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