SwifT4 Ligase Master Mix, 4X
ScienCell’s SwifT4 Ligase Master Mix (T4LIGMM) is a 4X premix that simplifies the reaction setup while also ensuring rapid ligation at room temperature. T4LIGMM is ideal for use with both sticky-end and blunt-end DNA and needs only 5 minutes for the ligation reaction. The 4X premix contains an optimized ratio of enzyme, buffer and transformation enhancer. As it maintains a liquid state during storage at -20°C, no thawing of the master mix is required. Simply combine T4LIGMM and DNA, the resulting reaction mixture may then be used directly for bacterial transformation.
Kit Components:
Catalog #MB6308-50
| Cat # | Item | Quantity | Storage |
| MB6308-50 | SwifT4 Ligase Master Mix, 4X | 150 µL | -20°C |
Catalog #MB6308-200
| Cat # | Item | Quantity | Storage |
| MB6308-200 | SwifT4 Ligase Master Mix, 4X | 600 µL | -20°C |
ScienCell’s SwifT4 Ligase Master Mix (T4LIGMM) is a 4X premix that simplifies the reaction setup while also ensuring rapid ligation at room temperature. T4LIGMM is ideal for use with both sticky-end and blunt-end DNA and needs only 5 minutes for the ligation reaction. The 4X premix contains an optimized ratio of enzyme, buffer and transformation enhancer. As it maintains a liquid state during storage at -20°C, no thawing of the master mix is required. Simply combine T4LIGMM and DNA, the resulting reaction mixture may then be used directly for bacterial transformation.
Quality Control
The performance of T4LIGMM is verified by transformation efficiency of recircularized vector.
| Catalog No. | MB6308 |
|---|---|
| Country of Manufacture | United States |
| Product Code | T4LIGMM |
| Size/Quantity | 50 reactions, 200 reactions |
| Product use | T4LIGMM is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Upon receipt, store SwifT4 Ligase Master Mix, 4X (Cat # T4LIGMM) at -20°C in a manual defrost freezer. Aliquot as needed. |
| Shipping | The product is shipped on dry ice. |
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