SARS-CoV-2 SYBR® Green RT-qPCR Quantification Kit
ScienCell's SARS-CoV-2 SYBR® Green qPCR Quantification Kit (SVSGQ) is designed to purify and quantify the SARS-CoV-2 coronavirus presence in samples.
Kit Components:
| Catalog # | Component | Quantity |
| MB6018a-1 | GoldNStart TaqGreen qPCR Master Mix | 1 mL x 4 |
| 7058a | SARS-CoV-2 N gene primer set, in solution | 200 μL |
| 7058b | SARS-CoV-2 S gene primer set, in solution | 200 μL |
| 7058c | Human ACE2 gene primer set, in solution | 200 μL |
| 7058d | Human ACTB gene primer set, in solution | 200 μL |
| GQ100-4 | Nuclease-free H2O | 4 mL |
| 7058-Pos | Positive control (non-infectious; RNA: 500 – 1000 copies/μL, cells: 200 – 300 counts/μL) |
200 μL |
For Research Use Only. Not for use in diagnostic procedures.
Note: When purchasing multiple kits, shipping costs may be higher than the estimate
ScienCell's SARS-CoV-2 SYBR® Green qPCR Quantification Kit (SVSGQ) is designed to purify and quantify the SARS-CoV-2 coronavirus presence in samples.
Four primer sets are included in the kit. Two of them (Cat #7058a and 7058b) target coronavirus SARS-CoV-2 nucleocapsid (N) gene and spike (S) gene, respectively. The human ACE2 gene primer set (Cat #7058c) targets human angiotensin-converting enzyme 2 (ACE2) gene, which serves as a cellular receptor for SARS-CoV-2. The human ACTB gene primer set (Cat #7058d) targets human β-actin (ACTB) housekeeping gene, which serves as a normalization control for cell number quantification. All primer sets are verified to possess high specificity and efficiency near 100%, with no primer dimer formation under recommended PCR conditions.
Additionally, a non-infectious positive control (Cat #7058-Pos) and nuclease-free water (Cat #GQ100-4) are included in the kit. The positive control (Cat #7058-Pos) consists of non-infectious RNA fragments spiked with human small airway epithelial cells and serves to ensure reagents and instruments are working properly.
| Catalog No. | RU7058 |
|---|---|
| Country of Manufacture | United States |
| Product Code | SVSGQ |
| Size/Quantity | 100 reactions. |
| Product use | For Research Use Only. Not for use in diagnostic procedures |
| Storage | Please refer to product sheet. |
| Shipping | The product is shipped on dry ice. |
No Publication available at this time.
