Recombinant Human Stem Cell Factor
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Stem Cell Factor (SCF) plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In in vitro and in vivo, SCF can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. On purified primitive human and mouse hematopoietic precursors, SCF acts in a synergistic manner with various growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and lymphoid lineage colony formation.
| Catalog No. | 102-01 |
|---|---|
| Product Code | rhSCF |
| Country of Manufacture | United States or China |
| Size/Quantity | 2ug, 10ug, or 1mg |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store at -20°C after receiving. Upon reconstitution, store at 2-8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Dry ice. |
| Synonyms | Mast Cell Growth factor (MGF), c-kit Ligand and Steel-Factor (SLF) |
| AA Sequence | MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTD LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids. |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. The ED50 is < 10ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg, as determined by proliferation of BAF3 cells expressing FGF receptors |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2µm filtered concentrated (0.5mg/ml) solution in PBS, pH 7.4. |
| Endotoxin | Less than 1EU/µg of rHuOPG-Fc as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |
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