Recombinant Human Hepatocyte Growth Factor
Recombinant Human Hepatocyte Growth Factor (rhHGF) is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Hepatocyte Growth Factor (HGF), also known as scatter factor or hepatopoietin A, is a pleiotropic cytokine belonging to the plasminogen-related S1 serine protease family. Structurally, HGF contains an N-terminal PAN/APPLE-like domain, four kringle domains, and a C-terminal serine protease–like domain lacking enzymatic activity. Human HGF is synthesized as an inactive 728 amino acid single-chain precursor that is proteolytically cleaved into an active disulfide-linked heterodimer consisting of an ~60 kDa α-chain and ~30 kDa β-chain. Alternative splicing generates multiple isoforms with truncated domain composition. HGF is highly conserved across mammalian species (>90% sequence identity).
HGF binds heparan sulfate proteoglycans and signals through the receptor tyrosine kinase c-MET. Activation of the HGF/c-MET pathway regulates cell proliferation, motility, and morphogenesis, and is implicated in tumor development and progression. Functionally, HGF promotes epithelial cell scattering and branching morphogenesis, partly through upregulation of integrin α2β1, a collagen I receptor. It also modulates cell-cell adhesion via induction of nectin-1 ectodomain shedding. In thyroid cells, HGF stimulates proliferation and migration while suppressing differentiation and TSH-dependent iodine uptake. Additionally, HGF enhances migration of cardiac stem cells in damaged myocardium.
| Catalog No. | 105-39 |
|---|---|
| Country of Manufacture | United States |
| Product Code | rhHGF |
| Size/Quantity | 10 ug, 100 ug and 500 ug |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, store at -20°C after receiving. Upon reconstitution, store at 2- 8°C for up to one week. For maximal stability, aliquot and store at -20°C. Avoid repeated freeze/ thaw cycles. |
| Shipping | Gel pack |
| Synonyms | Hepatocyte Growth Factor, HGF, DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF |
| AA Sequence | QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKIC QRWDHQTPHR HKFLPERYPD KGFDDNYCRN PDGQPRPWCY TLDPHTRWEY CAIKTCADNT MNDTDVPLET TECIQGQGEG YRGTVNTIWN GIPCQRWDSQ YPHEHDMTPE NFKCKDLREN YCRNPDGSES PWCFTTDPNI RVGYCSQIPN CDMSHGQDCY RGNGKNYMGN LSQTRSGLTC SMWDKNMEDL HRHIFWEPDA SKLNENYCRN PDDDAHGPWC YTGNPLIPWD YCPISRCEGD TTPTIVNLDH PVISCAKTKQ LR VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS |
| Source | mammalian cell |
| Molecular Weight | 53.7 kDa (alpha chain), 26 kDa (beta chain) |
| Purity | > 95% |
| Physical Appearance | Light yellow to white powder |
| Formulation | Lyophilized from a 0.2µm filtered solution in 20 mM Tris-HCl pH 7.5, 400 mM NaCl and Dextran as protectant. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water or 1x PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |
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