Ready-To-Use 3D Human Airway Spheroids

Airway remodeling, chronic inflammation, and bacterial colonization in many airway diseases occur in the lower respiratory tract. Primary bronchial epithelial cells are, therefore, an excellent in vitro source for studying airway diseases such as cystic fibrosis, chronic obstructive pulmonary disease (COPD), and severe asthma. The bronchial epithelium is made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. ScienCell's ready-to-use 3D Human Airway Spheroids (SP3D-HAS) are generated using primary bronchial epithelial cells and are embedded in their own secreted extracellular matrix (ECM). Importantly, immunofluorescence staining and qPCR analysis revealed the presence of basal, ciliated, and goblet cells in ScienCell's airway spheroids (see Figures 1 and 2). ScienCell's ready-to-use airway spheroids (Cat #SP3D-3210) are great models for studying airway diseases in a simple and high throughput manner.