One-Step GoldNStart TaqGreen RT-qPCR Master Mix, 4x
ScienCell's One-Step GoldNStart TaqGreen RT-qPCR master mix, 4x (GTGRTMM) is designed for sensitive RNA reverse transcription and quantification by SYBR® Green dye-based reverse transcription quantitative PCR (RT-qPCR). The ready-to-use 4x master mix contains UNG/dUTP (to eliminate PCR carryover contamination), engineered M-MLV reverse transcriptase with "tepid-start" property, Taq DNA polymerase with "hot-start" property, dNTPs, a buffer system for robust reverse transcription and qPCR, and an inert gold-color loading indicator (ScienCell, catalog #GQ300G).
ScienCell's One-Step GoldNStart TaqGreen RT-qPCR master mix, 4x (GTGRTMM) is designed for sensitive RNA reverse transcription and quantification by SYBR® Green dye-based reverse transcription quantitative PCR (RT-qPCR). The ready-to-use 4x master mix contains UNG/dUTP (to eliminate PCR carryover contamination), engineered M-MLV reverse transcriptase with “tepid-start†property, Taq DNA polymerase with “hot-start†property, dNTPs, a buffer system for robust reverse transcription and qPCR, and an inert gold-color loading indicator (ScienCell, catalog #GQ300G). The "tepid-start" and "hot-start" property achieved through ScienCell's unique chemically modified M-MLV reverse transcriptase and Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes.
| Catalog No. | MB6028 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GTGRTMM |
| Size/Quantity | 1 mL or 5 mL |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, store One-Step GoldNStart TaqGreen RT-qPCR Master Mix, 4x (Cat #MB6028a) at -20°C in a manual defrost freezer and nuclease-free H2O (Cat #MB6028b) at 4°C. Aliquot as needed. Avoid repeated freeze-and-thaw cycles. |
| Shipping | Dry ice. |
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