Mouse Neurotrophin-3 ELISA Kit
For quantitative detection of mouse Neurotrophin-3 in serum, body fluids, tissue lysates or cell culture supernatants.
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor (NGF) gene family, and plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
ScienCell's mouse Neurotrophin-3 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Neurotrophin-3-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse Neurotrophin-3 in the sample that is captured on the strips.
| Catalog No. | EK0473 | ||||||||||||||||||||||||||||||||||||||||||
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| Country of Manufacture | China | ||||||||||||||||||||||||||||||||||||||||||
| Product Code | mNT3-ELISA | ||||||||||||||||||||||||||||||||||||||||||
| Size/Quantity | 96 Tests in 8 x 12 divisible strips | ||||||||||||||||||||||||||||||||||||||||||
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. | ||||||||||||||||||||||||||||||||||||||||||
| Storage | Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. | ||||||||||||||||||||||||||||||||||||||||||
| Shipping | Gel ice. | ||||||||||||||||||||||||||||||||||||||||||
| Reactive Species | Mouse | ||||||||||||||||||||||||||||||||||||||||||
| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | cell culture supernatants and serum. | ||||||||||||||||||||||||||||||||||||||||||
| Clonality Of Antibodies | monoclonal antibody from rat|polyclonal antibody from goat | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Y139-T257 | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <2 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Assay Range | 15.6 pg/ml - 1,000 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Cross Reactivity | There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. | ||||||||||||||||||||||||||||||||||||||||||
| Kit Components | Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4 | ||||||||||||||||||||||||||||||||||||||||||
| Materials Required But Not Included In Kit | Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Data |
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| Data Example Image |
Mouse Neurotrophin-3 PicoKine ELISA Kit standard curve
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| Intra Assay Consistency Inter Assay Consistency |
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| Reproducibility |
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| Gene Name | NTF3 | ||||||||||||||||||||||||||||||||||||||||||
| Uniport ID / Gene ID | P20181/18205 | ||||||||||||||||||||||||||||||||||||||||||
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