Mouse BAFF ELISA Kit

B-cell activating factor (BAFF), also known as tumor necrosis factor ligand superfamily member 13B, is a protein that in humans is encoded by the TNFLSF13B gene. BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. All these ligands act as heterotrimers (i.e. three of the same molecule), interacting with heterotrimeric receptors, although BAFF has been known to be active as either a hetero- or homotrimer. The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with a molecular weight 23.2 kDa.

ScienCell's mouse BAFF ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse BAFF-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The mouse-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse BAFF in the sample that is captured on the strips.