Lipid Accumulation/ Steatosis Colorimetric Assay Kit

Hepatic steatosis is characterized by the excessive accumulation of fat, primarily in hepatocytes, leading to the formation of lipid droplets in the cytoplasm and are visible under a microscope. The ScienCell Steatosis Colorimetric Assay Kit (SCA) provides a convenient solution for evaluating the potential risk of steatosis associated with drug candidates. It utilizes Oil Red O to selectively stain neutral lipids within cells. Lipid accumulation can be quantified using a plate reader after extracting the dye from the lipid droplets. The kit includes oleic acid as a positive control, a commonly used substance in drug screening for steatosis. Additionally, this kit can be used to quantify lipid droplet formation in other cell types, such as adipocytes.