L-Lactate Assay
Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology.
Lactate is an important intermediary in glucose metabolism. Under hypoxic or anaerobic conditions, lactate dehydrogenase (LDH) converts pyruvate, the final product of glycolysis, to lactate. Monitoring lactate levels is an adequate indicator of the balance between tissue oxygen demand and utilization, and is useful when studying cellular physiology. This colorimetric assay is based on the reduction of the tetrazolium salt, INT, in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm. The intensity of the optical density is proportional to the lactate concentration.
| Catalog No. | 8308 |
|---|---|
| Country of Manufacture | United States |
| Product Code | LAC |
| Size/Quantity | 100 Tests in 96-well plate |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receipt, store the Assay Buffer (Cat. #8308a) and Reconstitution Solution (Cat. #8308e) at 4°C. Store L-Lactate Standard (Cat. #8308b), Enzyme Mix (Cat. #8308c), and Substrate Mix (Cat. #8308d) at -20°C. |
| Shipping | Dry ice. |
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