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Inorganic Pyrophosphatase (Yeast)
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate. PPase catalyzed reaction is Mg2+-dependent. PPase is used for in vitro transcription (IVT) to increase RNA synthesis yield by removing the inhibitory PPi. Yeast Inorganic Pyrophosphatase is heat-sensitive and loses activity rapidly above 55 °C, avoid prolonged exposure to elevated temperatures.
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