Human p53 ELISA Kit

p53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human p53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kDa protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. Human kidney and heart express only full-length p53. p53 plays a vital role in conserving stability by preventing genome mutation.

ScienCell's human p53 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human p53-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human p53 in the sample that is captured on the strips.