Human Calvarial Osteoblast Lysate
HCO Lysate is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Quantity: 200 μg
Human Calvarial Osteoblast Lysate (HCO Lysate) is prepared from early passage Human Calvarial Osteoblasts using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF). Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer. Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% β-mercaptoethanol). Lysate from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.
| Catalog No. | 4606 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCO Lysate |
| Size/Quantity | 200 ug |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store lysate immediately at -80°C upon arrival; aliquot the lysate to avoid repeated freeze/thaw cycles |
| Shipping | Dry ice. |
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