HIV-1 Virus One-step RT-PCR Detection Kit

HIV-1, human immunodeficiency virus 1, is a type of retrovirus that attacks the human immune system. If HIV-1 infection is not treated with anti-retroviral drugs, it can cause Acquired Immunodeficiency Syndrome, otherwise known as AIDS. There are four groups of HIV-1 virus: M, N, O, and P. The group M is further divided into nine subtypes: A, B, C, D, F, G, H, J, and K. ScienCell's HIV-1 Virus One-step RT-PCR Detection Kit (DHIV1) is designed to detect the presence of HIV-1 virus in cultured cells by one-step reverse transcription PCR (RT-PCR), followed by the electrophoresis analysis of the PCR product. The HIV-1 primer set (included in Cat #7168-ps) targets all four groups, including nine group M subtypes of HIV-1, and gives a positive band at 175 bp. The human control primer set (also included in Cat #7168-ps) targets human B2M gene RNA, but not genomic DNA, with a positive band of 85 bp and acts as a control to assess specimen quality. The positive control (Cat # RU011-pos) consists of noninfectious HIV-1 RNA fragment spiked into human primary cells and serves to ensure reagents and instruments are working properly. In addition, RubyNStart one-step RT-PCR master mix (Cat #MB6058a), RubyNStart enhancer (Cat #MB6058b), cell lysis buffer (Cat #GQ400a), cell lysis buffer enhancer (Cat #GQ400b), and nuclease-free water (Cat #GQ100-4) are included in the kit. Please refer to Tables 4 and 5 for results interpretation.