HiPSC-derived Endothelial Cells
Includes 1 vial of HiPSC-EC (Cat. #1690, 1 mL, >1 x 106 cells/vial), 500 mL Endothelial Cell Medium-basal (ECM, Cat. #1001), 5 mL Endothelial Cell Growth Supplement (ECGS, Cat. #1052), 25mL Fetal Bovine Serum (FBS, Cat. #0025), and 5mL Penicillin-Streptomycin (P/S, Cat. #0503)
Human Endothelial Cells (HiPSC-EC) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (HiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HiPSC efficiently generate mesoderm cells via small-molecule activation of WNT signaling in chemically defined media, and subsequently converted to endothelial cells through endothelial cell specification using vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF).
The derived endothelial cells are characterized by immunofluorescence with antibodies specific to CD31 and vWF. The cell population is highly pure: >80% of cells express CD31 (PECAM-1) & vWF positive. HiPSC-EC are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. Cells are negative for mycoplasma, bacteria, yeast and fungi. After reviving, HiPSC-EC can be maintained in Endothelial Cell Medium as an adherent culture. HiPSC-EC are not recommended for expanding or long-term cultures.
Recommended Medium
It is recommended to use the provided Endothelial Cell Medium (ECM, Cat. #1001) for plating HiPSC-EC and culturing them.
| Catalog No. | 1690 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HiPSC-EC |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer Cat. #1690 from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Store Cat. #1001 at 4°C, Cat. #1052, 0025 and 0503 at -20°C. |
| Shipping | Cat. #1690, 1052, 0025 and 0503 are shipped on dry ice. Cat. #1001 is shipped at room temperature. |
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