GeneQuery™ Human PI3K/AKT Signaling Pathway qPCR Array Kit

ScienCell’s GeneQuery™ Human PI3K/AKT Signaling Pathway qPCR Array Kit (GQH-AKT) profiles 88 key genes associated with the PI3K (Phosphoinositide 3-kinases)/Akt signaling pathway, which is an intracellular signal transduction pathway that promotes metabolism, proliferation, cell survival, growth and angiogenesis. In the pathway, PI3K is activated by many types of extracellular signals or toxic insults. Activated PI3K catalyzes the production of phosphatidylinositol-3,4,5-triphosphate (PIP3) at the cell membrane. PIP3 in turn serves as a second messenger that helps to activate Akt. Once active, Akt can control key cellular processes by phosphorylating substrates involved in apoptosis, protein synthesis, metabolism, and cell cycle. Brief examples of how included genes may be grouped according to their functions are shown below:

• PI3K and AKT Family Genes and Regulators: AKT1, AKT2, AKT3, BTK, GRB2, HSPB1, ILK, PDPK1, PIK3CA, PIK3CG, PIK3R1, PIK3R2, PAK1, PRKCA, PRKCB, PRKCZ, PTEN
• IGF/AKT Signaling: CSNK2A1, ELK1, FOS, GRB2, HRAS, IGF1, IGF1R, IRS1, JUN, MAP2K1, MAPK3, MAPK8, PTPN11, RAF1, RASA1, SHC1, SOS1, SRF
• AKT/mTOR Signaling: EIF4B, EIF4E, MTOR, PTEN, RHEB, RPS6KB1, TSC1, TSC2
• GSK3 Signaling Pathway: ADAR, APC, CCND1, CD14, CTNNB1, EIF2AK2, GJA1, GSK3B, IRAK1, MYD88, NFKB1, TIRAP, TLR4
• Cell Cycle Arrest and Apoptosis: CDKN1B, FASLG, FOXO3, GRB2, ILK, ITGB1, MAPK1, MAPK3, PTEN, PTK2, RBL2, SHC1, SOS1
• BAD Phosphorylation and Cell Survival: BAD, GRB2, HRAS, IGF1R, IRS1, MAP2K1, MAPK1, MAPK3, RAF1, RPS6KA1, SHC1, SOS1, YWHAH
• Cell Migration: CDC42, PDGFRA, RAC1, RHOA, WASL
• Other Downstream Signaling Pathways: EIF4E, MTOR, IRS1, MAPK1, MAPK14, MAPK3, PRKCA, PTEN, RPS6KB1
• Other Genes Involved in the AKT Signaling Pathway: CASP9, CHUK, FOXO1, NFKBIA, DPYSL2, GSK3A, FOXO4, MDM2, NOS1, NOS3, FOXA2, NR4A1, INPP4B, RPS6KB2, HTT, FKBP5, CREB1, IKBKB

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.