GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit
GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit (SBT-DPB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.
Kit Components:
| Cat # | Component | Quantity | Storage |
| GK894a | HLA-DPB1 amplification primer set (P_ampF and P_ampR) | 400 µL | -20°C |
| GK894b | P_2F sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK894c | P_2R sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK894d | P_3F sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK894e | P_3R sequencing primer mix, 10 µM | 200 μL | -20°C |
| GK894h | Nuclease-free H2O | 4 mL | 4°C |
| GK894r | Human genomic DNA reference (GDR), ~50 ng/µL (HLA-DPB1*01:01 and HLA-DPB1*02:01) |
10 μL | -20°C |
| MB6038-1 | 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL | 2 vials | -20°C |
-
This Item:
$739.00
-
First-Strand cDNA Synthesis Master Mix, 4x
$207.00
-
GoldNStart TaqGreen qPCR Master Mix
As low as $82.00
The human leukocyte antigen (HLA) complex is a gene complex located on the short arm of human chromosome 6. The HLA complex is the human version of the major histocompatibility complex (MHC) which helps the immune system distinguish host cells from invaders. It is the most polymorphic locus in the human genome, and has been implicated in many pathologies including autoimmunity disorders and cancer.
GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit (SBT-DPB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.
SBT-DPB1 kit contains a ScienCell proprietary HLA-DPB1 amplification primer set (P_ampF primer mix and P_ampR primer mix; Cat #GK894a) that can amplify a region in the human genome that covers exons 2-3 of HLA-DPB1 gene, and 4 sequencing primer mixes (P_2F, P_2R, P_3F, and P_3R; Cat #s GK894b-e) that can be used in the following Sanger sequencing of the PCR product. Please refer to Figure 2 for the binding location of the primers on the human genome. Each GeneQuery™ HLA typing kit contains a human Genomic DNA Reference (GDR) sample with known HLA alleles (Cat #GK894r). The GDR can be used to monitor the quality of the reagents and the instruments. The HLA-DPB1 alleles in GK894r are HLA-DPB1*01:01 and HLA-DPB1*02:01.
Primer binding locations on the human genome.
Additional Materials Required (Materials Not Included in Kit)
| Component | Recommended |
| genomic DNA template | Customers’ samples |
| PCR purification kit | SpeeDNA PCR Purification Kit (ScienCell, Cat #MB6948) |
| Sanger sequencing kit | |
| PCR plates or tubes | |
| Catalog No. | GK894 |
|---|---|
| Country of Manufacture | United States |
| Product Code | SBT-DPB1 |
| Size/Quantity | 40 samples |
| Product use | SBT-DPB1 is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
| Storage | Primers & EmeraldNStart HiFi Marathon PCR Master Mix at -20°C in a manual defrost freezer, nuclease-free H2O at 4°C. Once the EmeraldNStart HiFi Marathon PCR Master Mix is thawed, store at 4°C, do not refreeze. It's stable at 4°C for up to 6 months. |
| Shipping | Dry ice. |
No Publication available at this time.