Colorimetric GAPDH Assay
Colorimetric GAPDH Assay kit is used to evaluate GAPDH activity in vitro. This kit is for research use only and is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The ScienCell™ Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric acid (3-PGA), adenosine 5'-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time (ΔA340nm/min).
| Catalog No. | 8148 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GAPDH |
| Size/Quantity | 100 tests |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Refer to Product Sheet |
| Shipping | Dry ice. |
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