3D-Liver Spheroid Medium

3D-LSpM consists of 200 ml of basal medium, 4 mL of 3D-liver spheroid supplement (3DLSpS, Cat. #3D-5252) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3DLSpS and P/S are not pre-mixed in the 3D-LSpM; they must be added separately to make the complete medium.