3D-Kidney Spheroid Medium
3D-KSpM consists of 200 mL of basal medium, 10 mL of FBS (Cat. #0010), 2 mL of 3D-kidney spheroid supplement (3D-KSpS, Cat. #3D-4302), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-KSpS, FBS and P/S are not pre-mixed in the 3D-KSpM; they must be added separately to make the complete medium.
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3D-Kidney Spheroid Medium (3D-KSpM) is a specialized medium designed for the generation and maintenance of spheroids consisting of human primary renal proximal tubular epithelial cells, mesangial cells, and glomerular endothelial cells in vitro in conjunction with fetal bovine serum (FBS, Cat. No. 0010) and 3D-kidney spheroid supplement (3D-KSpS, Cat. #3D-4302). 3D-KSpM is a sterile, viscous medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
| Catalog No. | 3D-4301 |
|---|---|
| Country of Manufacture | United States |
| Product Code | 3D-KSpM |
| Size/Quantity | 200 mL of basal medium, 10 mL of FBS, 2 mL of 3D-KSpS, and 2 mL of P/S |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Store the basal medium at 4°C and the 3D-KSpS, FBS and P/S solution at -20°C. Protect from light. |
| Shipping | Basal medium: room temperature. Supplements: dry ice. |
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