3D-Hepatic Stellate-Endothelial Cell Spheroid Medium
3D-SECSpM consists of 200 ml of basal medium, 4 mL of 3D-sinusoidal endothelial cell spheroid supplement (3D-SECSpS, Cat. #3D-5452) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SECSpS and P/S are not pre-mixed in the 3D-SECSpM; they must be added separately to make the complete medium.
3D-Hepatic Stellate-Endothelial Cell Spheroid Medium (3D-SECSpM) is a specialized medium designed for the generation and maintenance of spheroids consisting of human primary hepatic stellate cells and sinusoidal endothelial cells in vitro in conjunction with 3D-sinusoidal endothelial cell spheroid supplement (3D-SECSpS, Cat. #3D-5452). 3D-SECSpM is a sterile, viscous medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
| Catalog No. | 3D-5401 |
|---|---|
| Country of Manufacture | United States |
| Product Code | 3D-SECSpM |
| Size/Quantity | 200 mL of basal medium, 4 mL of 3D-SECSpS, and 2 mL of P/S |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Store the basal medium at 4°C and the 3D-SECSpS and P/S solution at -20°C. Protect from light. |
| Shipping | Basal medium: room temperature. Supplements: dry ice. |
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