3D-Hepatic Stellate Cell Spheroid Medium
3D-SteCSpM consists of 200 ml of basal medium, 4 mL of FBS (Cat. #0004), 2 mL of 3D-stellate cell spheroid supplement (3D-SteCSpS, Cat. #3D-5352) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-SteCSpS, FBS and P/S are not pre-mixed in the 3D-SteCSpM; they must be added separately to make the complete medium.
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3D-Hepatic Stellate Cell Spheroid Medium (3D-SteCSpM) is a specialized medium designed for the generation and maintenance of spheroids consisting of human primary hepatic stellate cells in vitro in conjunction with fetal bovine serum (FBS, Cat. #0004) and 3D-stellate cell spheroid supplement (3D-SteCSpS, Cat. #3D-5352). 3D-SteCSpM is a sterile, viscous medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
| Catalog No. | 3D-5301 |
|---|---|
| Country of Manufacture | United States |
| Product Code | 3D-SteCSpM |
| Size/Quantity | 200 mL of basal medium, 4 mL of FBS, 2 mL of 3D-SteCSpS and 2 mL of P/S |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Store the basal medium at 4°C and the 3D-SteCSpS, FBS and P/S solution at -20°C. Protect from light. |
| Shipping | Basal medium: room temperature. Supplements: dry ice |
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