3D-Blood Brain Barrier Spheroid Medium

3D-BBBSpM consists of 200 ml of basal medium, 10 mL of FBS (Cat. #0010), 2 mL of 3D-blood brain barrier spheroid supplement (3D-BBBSpS, Cat. #3D-8752), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-BBBSpS, FBS, and P/S are not pre-mixed in the 3D-BBBSpM; they must be added separately to make the complete medium.