RPMI 1640 培养基（含 L-谷氨酰胺和 HEPES）

RPMI 1640 media were originally developed for suspension cultures or monolayer cultures of human leukemia cells. In addition, they intended to be used with a serum supplement. Recently, RPMI-1640 media have been shown to support several cell lines in the absence of serum. They have also been widely used in fusion protocols and in the growth of hybrid cells. HEPES buffer is included in the formulation at a final concentration of 25 mM to compensate for the loss of buffering capacity incurred by eliminating serum. RPMI 1640 with L-Glutamine and HEPES (RPMI 1640) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones. It is well suited for supporting the growth of a broad range of mammalian cell lines. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

RPMI 1640 培养基最初开发用于人白血病细胞的悬浮培养和单层培养，并设计为配合血清补充剂使用。近年来，研究表明 RPMI 1640 培养基在无血清条件下同样能够支持多种细胞系的生长。此外，该培养基还广泛应用于细胞融合实验和杂交细胞培养。为弥补去除血清后缓冲能力的降低，配方中添加了终浓度为 25 mM 的 HEPES 缓冲剂，以增强培养基的 pH 稳定性。

RPMI 1640 培养基（含 L-谷氨酰胺和 HEPES）（RPMI 1640）是一种无菌液体培养基，含有必需氨基酸和非必需氨基酸、维生素、有机和无机化合物以及激素等成分，适用于支持多种哺乳动物细胞系的生长。

本产品仅供科研使用，不得用于人体、动物或体外诊断用途。