人线粒体DNA拷贝数相对定量 qPCR 检测试剂盒

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases. ScienCell's Relative Human Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (RHMQ) is designed to directly compare the average mtDNA copy number of the samples. The mtDNA primer set recognizes and amplifies one of the most conserved regions on human mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17 and serves as reference for data normalization. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

Kit Components:

Cat #	Component	Quantity	Storage
MB6018a-1	2X GoldNStart TaqGreen qPCR master mix, 1 mL	2 vials	-20°C
8938a	Human mtDNA primer set, lyophilized	1 vial	-20°C
8938b	Single copy reference (SCR) primer set, lyophilized	1 vial	-20°C
8938c	Nuclease-free H2O	4 mL	4 °C

线粒体DNA（mtDNA）是一种位于线粒体中的环状、多拷贝基因组DNA。线粒体作为细胞内的重要细胞器，在细胞能量产生过程中发挥关键作用。细胞的能量生成能力取决于mtDNA的完整性及其拷贝数。大量研究表明，mtDNA拷贝数的变化与衰老以及多种与年龄相关的疾病密切相关，例如癌症、糖尿病和神经退行性疾病。

ScienCell 人线粒体DNA拷贝数相对定量 qPCR 检测试剂盒（RHMQ）用于直接比较不同样品中mtDNA的平均拷贝数。该试剂盒中的mtDNA引物组可识别人类mtDNA上高度保守的区域并进行扩增，且不会扩增核基因组DNA中的非特异性序列。单拷贝参考基因（SCR）引物组针对人类第17号染色体上一段约100 bp的区域进行扩增，作为数据标准化的内参。精心设计的引物可确保：（i）高扩增效率，从而获得可靠的定量结果；（ii）无非特异性扩增。每组引物均通过qPCR熔解曲线分析和凝胶电泳验证其扩增特异性，并通过模板梯度稀释实验验证其扩增效率。

试剂盒组成：

货号	组分	规格	储存条件
MB6018a-1	2X GoldNStart TaqGreen qPCR 预混反应液，1 mL	2 管	-20°C
8938a	人 mtDNA 引物组（冻干粉）	1 管	-20°C
8938b	单拷贝参考基因（SCR）引物组（冻干粉）	1 管	-20°C
8938c	无核酸酶水（Nuclease-free H2O）	4 mL	4 °C