Recombinant Rat Fibroblast Growth Factor-basic
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Basic Fibroblast Growth Factor (bFGF) is a member of the FGF family of mitogenic peptides which is comprised of at least 22 proteins. FGF signaling factors play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. bFGF is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. bFGF signals through FGF receptor 1b, 1c, 2c, 3c and 4.
| 目录编号 | 144-02 |
|---|---|
| 产品编码 | rrbFGF |
| 制造国家 | United States or China |
| 规格/数量 | 10ug, 50ug, 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请储存于 -20°C。复溶后，可在 2-8°C 保存最多一周。为获得最大稳定性，请分装并储存于 -20°C。避免反复冻融。 |
| 运输 | 干冰。 |
| Synonyms | FGF-2, Fibroblast Growth Factor-basic |
| AA Sequence | PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKYSSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS |
| Source | Escherichia coli |
| Molecular Weight | Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 145 amino acids. |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active. The ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg, as determined by proliferation of BALB/c 3T3 cells |
| Physical Appearance | White lyophilized powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4. |
| Endotoxin | < 1EU/μg of growth factor as determined by LAL method. |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |