Recombinant Protein A/G
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Protein G similar to Protein A, is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria but with different specificities. It binds to the constant region of many species of immunoglobulin G and can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Individual subclasses of mouse monoclonals are most likely have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone. Optimal pH 5-8.
| 目录编号 | 1003-01 |
|---|---|
| 制造国家 | 中国 |
| 产品编码 | rProteinA/G |
| 规格/数量 | 5mg, 20mg, or 1g |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 复溶后保存在 2-8°C，最多一周。为获得最佳稳定性，请分装并保存在 -20°C。避免反复冻融。 |
| 运输 | 凝胶冰袋。 |
| Synonyms | Protein A/G, r-protein A/G, protein a/g, protein-a/g, protein-ag, protein ag. |
| Source | Escherichia coli |
| Molecular Weight | Approximately 56.9 kDa, consisting of 6xHis-tag on C-terminus and 8 IgG-binding domains EDABC (Protein A)-C1C2C3 (Protein G). |
| Purity | > 95% by SDS-PAGE |
| Physical Appearance | White lyophilized powder |
| Formulation | Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water or saline. |